RAW 264.7-EGFP cell line

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RAW 264.7-EGFP Order

RAW 264.7-EGFP cell line
Catalog#
YC-C020-EGFP-P
Size
1*10^6
Transfection Culture Medium
Transfection Culture Medium-C
Storage conditions
Shipped on dry ice; Store in liquid nitrogen
Datasheet
RAW 264.7-EGFP cell line
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Detailed Product Information

Product category
EGFP Stable Cell Lines
cell name
RAW 264.7-EGFP
Cell Morphology
monocyte/macrophage,adherent
Passage ratio
1:2-1:3
Species
Mouse
Reporter gene
EGFP
Resistance gene
puro
Construction method
Lentivirus
Mycoplasma test
negative
Culture method
90%DMEM+10%FBS
Accession
CVCL_C8WQ(Cellosaurus)
ExPASy Cellosaurus Database
Is a valuable online resource that compiles detailed information on over 100,000 cell lines from reputable cell banks such as ATCC, ECACC, and DSMZ. The database provides information including descriptions, origins, and characteristics of the cell lines, with broad reference value and scientific reliability. Additionally, the database provides information on cell line authentication, STR profiles, and potential contamination risks, helping researchers ensure the trustworthiness of the cell lines used in their experiments.
STR Profile
Click to download the STR appraisal report
Loci STR Info (Sample Cell)
Sample Cell Line:RAW 264.7		 STR Info (Cell bank)
Cell Line:RAW 264.7
Allele1Allele2Allele3Allele4Allele1Allele2Allele3
18-3 18.018.0
4-2 22.322.3
6-7 12.012.0
19-2
1-2 17.0
7-1 25.225.2
8-1 13.013.0
1-1 15.016.0
3-2 14.014.0
2-1 16.016.0
15-3 22.322.3
6-4 18.018.0
13-1 16.216.2
11-2 17.017.0
17-2 14.016.014.016.0
12-1 16.016.0
5-5 14.014.0
X-1 24.024.0
TH01
D5S818
* STR authentication data of this cell line matches with that of cell lines sourced from ATCC, DSMZ, JCRB, and RIKEN databases.
Conclusion: The STR identification of this cell is correct.

Instruction

Use EGFP gene expressing lentivirus constructed by Ubigene to infect the RAW 264.7 cell line， screen the cells based on the puromycin resistance marker and the optimal antibiotic screening concentration explored in advance, resulting in a RAW 264.7-EGFP cell line that can maintain long-term, stable and high expression.
RAW 264.7-EGFP cell line
RAW 264.7-EGFP
Catalog#
YC-C020-EGFP-P
Size
1*10^6
Storage conditions
Shipped on dry ice; Store in liquid nitrogen
Order

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